Pengaruh perbandingan konsentrasi Lactobacillus plantarum dan gula terhadap karakteristik cuka kulit lemon (Citrus limon)
Abstract
Lemon peel has various benefits and can be processed into higher-value products such as vinegar. Generally, vinegar is an acidic compound composed of acetic acid produced through a two-stage fermentation process. However, in this study, an innovation was introduced to convert acetic acid into lactic acid using Lactobacillus plantarum as the fermenting agent and sugar as the nutrient source. The aim of this study was to determine the effect of varying concentrations of L. plantarum and sugar on the chemical, physical, microbiological, and organoleptic characteristics of lemon peel vinegar. This study employed a Randomized Complete Block Design (RCBD) consisting of two factors: L. plantarum concentration (5%, 10%, 15%) and sugar concentration (15%, 10%, 5%). The results showed that the concentration ratio of L. plantarum and sugar significantly affected several characteristics of the lemon peel vinegar product. The best treatment was found in treatment A3, which used 15% L. plantarum and 5% sugar, with the following characteristics: moisture content of 93.43%, vitamin C content of 41.61 mg/100g, total titratable acidity (TTA) of 0.97%, pH of 2.76, viscosity of 3.72 cP, total plate count (TPC) of 13.75×10⁷ CFU/ml, and organoleptic test scores of 2.66 for color preference, 2.74 for aroma, 2.53 for taste, and 2.68 for texture.
References
AOAC. (2005). Official Methods of Analysis of the Association of Official Analitycal of Chemists. Association of Official Analitycal Chemist, Inc.
AOAC. (2007). Official Methods od Analysis of Association of Official Analytical Chemist. Association of Official Analitycal Chemist, Inc.
Ardilla, Y. A., Anggreini, K. W., & Rahmani, T. P. D. (2022). The role of indigenous lactic acid Bacteria Genus Lactobacillus in the fermentation process of Durian (Durio zibethinus) for Tempoyak production. Berkala Ilmiah Biologi, 13(2), 42–52. https://doi.org/10.22146/bib.v13i2.4619
Badan Standarisasi Nasional (BSN) RI. (2006). Petunjuk Pengujian Organoleptik dan atau Sensori.
Daryono, D., Elvianto, & Reksa, R. (2023). Ekstraksi Minyak Atsiri Kulit Jeruk Lemon (Citrus limon (L.) Burm.f.) dengan Pretreatment Microwave dan Distilasi Air-Uap. Jurnal Teknik Kimia USU, 12(2), 116–123. https://doi.org/10.32734/jtk.v12i2.12923
Dona, R., Ningrum, T. E. W., Hamzah, F., & Wahyuni, D. T. (2024). Penentuan Kadar Asam Asetat Dalam Larutan Cuka Makan Yang Beredar Di Pekanbaru Dengan Metode Alkalimetri.
Fajri, R. F., & Pato, U. (2021). Pemanfaatan Lactobacillus Plantarum 1 RN2-12112 dalam Pembuatan Minuman Sinbiotik Berbasis Sari Umbi Bengkuang dan Naga Merah. 8.
Febriani, D. R., & Azizati, Z. (2018). Pembuatan Cuka Alami Buah Salak Dan Pisang Kepok Beserta Kulitnya Teknik Fermentasi. Walisongo Journal of Chemistry, 1(2), 72–77. https://doi.org/10.21580/wjc.v2i2.3105
Hadiprawoto, Lukman, A., & Murtini, E. S. (2024). Studi Halal Dan Pengukuran Residu Alkohol Pada Beberapa Merek Cuka Apel [Sarjana, Universitas Brawijaya]. https://repository.ub.ac.id/id/eprint/216396/
Hidayatulloh, A., Gumilar, J., & Harlia, E. (2019). Potensi Senyawa Metabolit Yang Dihasilkan Lactobacillus Plantarum Atcc 8014 Sebagai Bahan Biopreservasi Dan Anti Bakteri Pada Bahan Pangan Asal Hewan. JITP, 7(2).
Lestari, I. N., Aina, G. Q., & Rica, F. N. (2023). Gambaran Kadar Vitamin C Pada Minuman Sari Lemon (Citrus Limon) Dengan Metode Spektrofotometri Uv – Vis Di Kota Samarinda. 3.
Noor, Z., Cahyanto, M. N., Indrati, R., & Sardjono, S. (2018). Skrining Lactobacillus plantarum Penghasil Asam Laktat untuk Fermentasi Mocaf. Agritech, 37(4), 437. https://doi.org/10.22146/agritech.18821
Nurhartadi, E., Nursiwi, A., Utami, R., & Widayani, E. (2018). Pengaruh Waktu Inkubasi Dan Konsentrasi Sukrosa Terhadap Karakteristik Minuman Probiotik Dari Whey Hasil Samping Keju. Jurnal Teknologi Hasil Pertanian, 11(2), 73. https://doi.org/10.20961/jthp.v11i2.29056
Shiyan, S., Pratiwi, G., Sari, A. R., & Alta, U. (2022). Narative Review: Profil Fitokimia Dan Potensi Farmakologi Citrus limon. Jurnal ’Aisyiyah Medika, 7(2). https://doi.org/10.36729/jam.v7i2.851
Tirtasatya Farrel Ricardo, Bambang Sigit Sucahyono, dan Yuyun Yuniati. (2025). Perbandingan Jus Kundur (Benincasa hispida) dan Penggunaan Gelatin terhadap Mutu Kimia dan Organoleptik (jelly drink). Composite : Jurnal Ilmu Pertanian. Vol. 7 No. 1 Februari 2025, Hlm: 50-58 DOI: https:/doi.org/10.37577/composite.v7i1.847
Utami, C. R. (2018). Karakteristik Minuman Probiotik Fermentasi Lactobacillus Casei Dari Sari Buah Salak. Teknologi Pangan: Media Informasi dan Komunikasi Ilmiah Teknologi Pertanian, 9(1). https://doi.org/10.35891/tp.v9i1.932
Wulandari, F., Nazaruddin, & Amaro, M. (2021). Pengaruh Jenis Bakteri Asam Laktat Dan Lama Fermentasi Terhadap Mutu Fisik, Kimia, Organoleptik Dan Mikrobiologi Tepung Mocaf. Prosiding Saintek, 3, 169–181.
Yusmarini, Y., Johan, V. S., Fitriani, S., Riftyan, E., & Siagian, O. M. (2021). Pemanfaatan Lactobacillus plantarum 1 RN2-53 dalam Pembuatan Minuman Probiotik Berbasis Sari Buah Melon dengan Variasi Penambahan Sukrosa. Jurnal Teknologi dan Industri Pertanian Indonesia, 13(1), 21–26. https://doi.org/10.17969/jtipi.v13i1.18531







1.png)



